Immunofluorescence analysis confirmed
myc tagged Notch1LNG was detected at the membrane as expected (Figure 2d, inset). Furthermore, by immunoblot analysis, both myc tagged constructs were expressed at comparable levels with increased amounts of processed NICD (85 generated in Notch1LNG CCSS transfected cells (Figure 2d inset). Cells undergoing neglect induced apoptosis showed a loss of adherence to substrate, which was apparent in the rounded morphology of cells undergoing neglect and disorganization of mitochondria (Figure 2e, GFP).
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